JaMBW Chapter 2.3
- ID: Sequence identifier
- RAW sequence
- TRA translation table, one-letter AA symbol, three-letter AA symbol, codons. Each AA line is
divided using the pipe symbol. Each entry in the AA line should be separated by a plus.
With this Applet, given a DNA sequence and a translation table, it is obtained the Protein translated code.
Q. How to use different translation tables
In order to modify the translation table, just read
this file using a word processor and modify the given translation table. Then save the file
and reload it in the WWW Browser you are using to read it.
Q. How to translate a DNA sequence to Protein
Just paste in the top window your sequence, or type it in manually. Then press the
"return" or "enter" button on your keyboard. Automagically the translated protein sequence
will appear in the window beneath.
HINT. What happens by adding One base at the beginning of the sequence ?
In order to see the effect of a single base insertion, type in front of the sequence
a single base, ( A, T, C or G) and then look at the resulting protein sequence. Try then
to answer at the questions:
Repeat the previous experiment inserting one, two, three and more bases, and try to answer
again at the previous questions. In addition try to answer:
- Is the new sequence equal to the previous one ?
- If yes why ?
- If no why ?
- Is there any "repeating pattern" in the results that you obtain ?
- If so, how does it compare with the "codon concept" ?
- How would it change if the DNA/RNA/Protein code would be be different ?
- What about codons of 1, 2, or 4, 5, 6 letters ?
- What good does the redundancy of the code ? What if would be smaller or larger ?
- How many aminoacids exist ? How many of them are "standard" ?
- How many nucleic acid bases ? How are they called ? What's their difference ?
Dna<->RNA<-> Protein translation is a bridge which links together huge area of Science.
Therefore from this Applet you can interactively explore the intricacies of the Open
Reading Frames (look at the ORFSeeker), and as well you can move to the Protein Universe,
and just by copy/pasting the product of the translation in the Isoelectric Point engine,
see how macromolecules with completely different biochemical behaviour can be obtained by a simple shift of one base ... and what happens with insertions and deletions !