GRIFFITH UNIVERSITY

Faculty of Science and Technology

School of Science

SS13050 (SS13BMM) BACTERIOLOGY

SPECIAL END-OF-SEMSTER EXAMINATION

December, 1992.


Question 1

Answer both questions.

(a) This question is composed of six (6) parts, all of which are to be answered.

(i) Why are 16S rRNAs thought to be the best molecules for undertaking phylogenetic studies ?

(ii) What major evolutionary findings have emerged from the study of 16S rRNA sequences ?

(iii) Describe and discuss the methods that have been used for studying 16S rRNA as a phylogenetic tool.

(iv) What is phylogenetic staining ?

(v) What are signature sequences and of what phylogenetic value are they ?

(vi) Describe and discuss the structure of 16S rRNA with respect to their functions in a bacterial cell.

(b) This question is composed of four (4) parts, all of which are to be answered.

(i) The anatomical and physiological features of the gastrointestinal tract of humans are different to that of the rumen of ruminants. Describe the ways by which they influence the microbial populations.

(ii) Discuss the host-microbe relationship that exists in the gastrointestinal tracts and the rumen. How does this relationshipdiffer from that observed in hydrothermal vent animal communities ?

(iii) Discuss the prospects and constraints of establishing genetically engineered microbes in the rumen.

(iv) "Microbes in the gastrointestinal tracts of humans are under constraints." Explain what these constraints are citing examples.

(46 marks)



Question 2

Write notes on and/or draw schematic diagrams for all nine (9) of the following

(a) Dental plaque, cavities and periodontal disease

(b) Archael nutrition

(c) Skin microflora

(d) Synergism

(e) Nitrogen cycling

(f) Probing for microbes in the environment

(g) Microbial community structure

(h) Soil microflora

(i) Microbial leaching of minerals

(54 marks)



Question Three

Write an essay on the comparative biology of the Archea, with reference to the Bacteria and Eucarya.

(30 marks)



Question Four

Write notes on all three (3) of the following: (a) The role of the Entner - Doudorof Pathway in the Archaea. In particular outlining the Atwo alternative variations of this pathway that have been indentified in the organisms.

(b) Energy production in the Methanogens.

(c) The role of the citric acid cycle enzymes in the metabolism of the Archaea.

(30 marks)


ss12bmi
Author: Dr Bharat Patel <B.Patel@griffith.edu.au>
HTML'd by Bharat Patel & Troy Baalham
[Created: 08 Jan 1996]
[Modified: 27 Sep 1996]