Curriculum Vitae - Bharat Patel
Research Experience and Current Research Interest
- Screening of bacteria producing, and estimation of enzymes,
(proteases, amylases, b-galactosidase), and fermentation products
(penicillin, citric acid, acid, lactic acid, ethanol)
- Scale up for enzyme production from lab to 600 litres.
- Use of continuous flow and industrial sharples centrifuges.
Bacteriology including Anaerobic Bacteriology:
- Isolation, cultivation and identification of pathogenic bacteria.
- Isolation and characterization of thermophilic aerobes.
- Isolation, identification and characterization of thermophilic
carbohydrate fermenters, acetogens, sulfate-reducers and archae
-bacteria (sulfur-metabolizing and methanogens).
- Electron microscopy (negative staining, thin sections).
- Developement of anaerobic culturing techiques.
- Setting up & use of GLC (TCD and FID), HPLC and FPLC to detect,
identify and quantitate fermentation products. Packing of columns.
- Physiology of anaerobes.
- Continuous culture of carbohydrate fermenting anaerobes.
Virology, Immunology and Cell culture:
- Propogation and preservation of various cell lines (Hela, MDCK, MBCK,
Hela 229, HEP-2, L3T3, NS1, SP2/0).
- Establishment of primary myoblast cultures from muscle of adult
- Preparation of polyclonal antibodies in rats, mice and rabbits.
- Production of viruses in cell cultures, suckling mice, and eggs.
- Purification of influenza viruses using CsCl and sucrose gradients
and with controlled pore glass (CPG) chromatography.
- Gel chromatography for protein purification.
- Gel electrophoresis of proteins and DNA.
- Estimation of proteins, sugars, DNA, RNA.
- Estimating skin and bacterial lipases using fluorometric methods.
- Radioactive tracer experiments to determine growth rates of
thermophilic bacteria in situ.
- Use of immunofluorescence methods to determine microbial growth rates
- Isolation ot phage from thermal environments.
- Determination of sulfides and proteins in hot springs.
- Developement of EM methods to study bacterial morphotypes in situ.
- Molecular ecological studies using 16S rRNA as the target molecules.
- Sulfur and phosphate utilizing bacteria in soil and their importance
- Growth hormones and their effect on ovine primary myoblasts in vitro.
Molecular Biology and Microbial Biochemistry:
- Purification of DNA, G+C determination.
- Manipulating DNA of fungi (S. cerevisiae and Fusarium, bacteria (B.
subtilis, E.coli, Sulfolobus, and T. aquaticus), animal cells and
Lamda Phage DNA oligomers in agarose plugs.
- Use of CHEF, FIGE and OFAGE pulse field gel electrophoresis to
separate large chromosome sized DNA.
- Application of newly developed monoclonal antibody methods to produce
monoclonals to agricuturally important antigens. Monoclonals produced
against fungal toxins, bacteria, virus, animal growth promotants and
- Gel electrophoresis and purification of plasmids.
- DNA hybridization.
- Amplification, cloning & sequencing of 16S rRNA bacterial genes.
- Amplification, cloning & sequencing of 18S rRNA genes from anaerobic fungi.
- Enzymic studies including cloning of genes of polysaccharide
degrading enzymes from thermophilic bacteria (CGTases, pullulanases,
amylases, DNA polymerases, dextranases, xylanases, proteinases and
- DNA sequencing using the ABI system.
Use of computers in microbiology:
- Use of IBM PC and Sun for phylogenetic analysis. Some computer
programing knowledge. Extensive use of packages such as GDE, clustalv,
Phylip, Mega, Diplo, Treecon, various alignment editors etc.
Author: Dr Bharat Patel
HTML'd by Troy Baalham
[Created 06 Sept 1995]
[Modified 06 Sept 1995]